Active transport of protons and electrons driven by redox enzyme reactions via a cofactor shuttle (NADH/NAD), a model: Kinetics of activity and evolution of redox potential gradients
Identifieur interne : 000126 ( France/Analysis ); précédent : 000125; suivant : 000127Active transport of protons and electrons driven by redox enzyme reactions via a cofactor shuttle (NADH/NAD), a model: Kinetics of activity and evolution of redox potential gradients
Auteurs : G. Demmano [Cameroun, France] ; E. Selegny [Cameroun, France] ; J. C. Vincent [France]Source :
- Bioelectrochemistry and Bioenergetics [ 0302-4598 ] ; 1992.
Abstract
In order to explore the concept and kinetics of electron and proton transport induced by redox gradients, a model of active transport of protons and electrons through an artificial membrane was designed. The pump is based on two inverse redox reactions catalysed by different dehydrogenases on each side of the membrane; these reactions are linked together by electrons and protons transported via a constantly regenerated NADH/NAD transmembrane shuttle. Firstly, steady state equations were developed for processes controlled by diffusion or reaction rates. Secondly, experiments showed the transfer of protons and electrons from glyceraldehyde-3-phosphate on one side of the membrane to lactate on the other side of the membrane. Numerical simulation using diffusion-reaction kinetics agrees quantitatively with the experimental results and describes the case of an irreversible reaction driving the redox potential difference and the system through three stages (induction, quasi-stationary state and termination) of time-evolution. Finally, extensive computations simulated systems driven by a redox potential gradient with reversible enzyme reactions (as in biology). The dependence of the pumping on membrane permeability and selectivity and on carrier concentration is shown, the evolution of the process and of the potentials being governed by the relative rates of transport and enzyme reactions. Asymmetries, factors affecting the system through modulation of enzyme activity and extensions of the model are discussed.
Url:
DOI: 10.1016/0302-4598(93)86113-F
Affiliations:
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The pump is based on two inverse redox reactions catalysed by different dehydrogenases on each side of the membrane; these reactions are linked together by electrons and protons transported via a constantly regenerated NADH/NAD transmembrane shuttle. Firstly, steady state equations were developed for processes controlled by diffusion or reaction rates. Secondly, experiments showed the transfer of protons and electrons from glyceraldehyde-3-phosphate on one side of the membrane to lactate on the other side of the membrane. Numerical simulation using diffusion-reaction kinetics agrees quantitatively with the experimental results and describes the case of an irreversible reaction driving the redox potential difference and the system through three stages (induction, quasi-stationary state and termination) of time-evolution. Finally, extensive computations simulated systems driven by a redox potential gradient with reversible enzyme reactions (as in biology). The dependence of the pumping on membrane permeability and selectivity and on carrier concentration is shown, the evolution of the process and of the potentials being governed by the relative rates of transport and enzyme reactions. Asymmetries, factors affecting the system through modulation of enzyme activity and extensions of the model are discussed.</div></front></TEI><affiliations><list><country><li>Cameroun</li><li>France</li></country><region><li>Haute-Normandie</li><li>Région Normandie</li></region><settlement><li>Rouen</li></settlement><orgName><li>Université de Rouen</li></orgName></list><tree><country name="Cameroun"><noRegion><name sortKey="Demmano, G" sort="Demmano, G" uniqKey="Demmano G" first="G." last="Demmano">G. Demmano</name></noRegion><name sortKey="Selegny, E" sort="Selegny, E" uniqKey="Selegny E" first="E." last="Selegny">E. 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